[The in vivo influence of gibberellic acid (GA3) on the phagocytic properties of peritoneal macrophages (author's transl)]

Abstract

Different concentrations of gibberellin acid GA3 - 10.0 mg, 1.0 mg and 0.1 mg - were applied intraperitoneally to white mice of the SPF strain on four consecutive days. Following the last application we injected E. coli, P. aerug. and Pr. vulg. intraperitoneally into the peritoneum of these animals, in a quantity of 1.5-2.0 X 10(6)/0.5 ml of sterile phosphate buffer. The peritoneal macrophages obtained by puncturing the peritoneum of the animals killed under chloroform anaesthesia showed, as compared with those of the control animals statistically most significantly higher values (P < 0.001) of the phagocytic factor and phagocytic index as well as of the activity of the acid phosphatase. In the test animals which received gibberellin acid, the inhibition of the lysosomal activity of peritoneal macrophages as a result of the application of trypan blue did not reach the level which was found to exist in the control animals and those infected with B CG - Praha.