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. 1980;39(6):665-70.

Visualization of rat brain glycoproteins in polyacrylamide gels by means of concanavalin A-peroxidase

  • PMID: 7456929

Visualization of rat brain glycoproteins in polyacrylamide gels by means of concanavalin A-peroxidase

W Pohle et al. Acta Biol Med Ger. 1980.

Abstract

A reliable method for visualization of mannose-containing glycoproteins in electrophoretically (SDS-PAGE) separated Tris-soluble, Triton-soluble and Triton-resistant fractions obtained from rat hippocampus, brain region supposed to play an essential role in learning and memory processes, was elaborated. The method is based on the Concanavalin A-horseradish peroxidase reaction applied successfully for the purpose of light and electron microscopic visualization of cellular carbohydrate components.

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