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. 1980 Aug 30;56(16):1666-72.

[Fluorometric DNA assay at nanogram levels in biological material]

[Article in Italian]
  • PMID: 7459087

[Fluorometric DNA assay at nanogram levels in biological material]

[Article in Italian]
C F Cesarone et al. Boll Soc Ital Biol Sper. .

Abstract

A high degree of reproducibility was obtained using a simple and rapid microfluorimetric method of DNA determination. The described method evaluates the increased fluorescence after reaction of 33258 Hoechst fluorochrome with calf thymus DNA and mouse liver chromatin preparations. The routinely used preparation reagents and the subcellular components do not interfere with the reaction.

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