Immunobiological behaviour of rabbit precipitating and non-precipitating (co-precipitating) antibodies
- PMID: 7461708
- PMCID: PMC1458152
Immunobiological behaviour of rabbit precipitating and non-precipitating (co-precipitating) antibodies
Abstract
Complement fixing capacity, antibody-dependent cell cytotoxicity and in vivo opsonic function of rabbit anti-DNP precipitating and co-precipitating antibodies were analysed. Precipitating antibody activated the complement system, but co-precipitating antibody did not. Both antibody preparations behaved similarly in antibody-dependent cytotoxicity reactions. When chicken erythrocytes were employed as target cells, both antibodies were inactive with homologous (rabbit) lymphocytes as effector cells but were active with heterologous lymphoid effector cells (human, guinea-pig). Clearance of antigen from the blood of mice was accelerated following injection of an optimal dose of precipitating antibody but co-precipitating antibody was ineffective even at different doses. Competition between precipitating and co-precipitating antibodies in complement fixation and antigen elimination following interaction with antigen successively or at the same time was investigated. In these tests, both antibodies competed with antigen by mass but not by affinity. The possibility exists that co-precipitating antibody has one high and one low affinity binding site and thus acts as an univalent blocking antibody and is therefore not able to form active complexes with antigen. The possible role of co-precipitating antibody in some humoral and cellular immune mechanisms is discussed.
Similar articles
-
Structure of asymmetric non-precipitating antibody: presence of a carbohydrate residue in only one Fab region of the molecule.Immunology. 1986 Feb;57(2):311-7. Immunology. 1986. PMID: 3081439 Free PMC article.
-
Interactions between precipitating and nonprecipitating antibodies in the formation of immune complexes.J Immunol. 1987 Apr 15;138(8):2587-92. J Immunol. 1987. PMID: 3559208
-
[Complement fixation ability of precipitating and co-precipitating antibodies of various animal species].Rev Argent Microbiol. 1979 Jan-Apr;11(1):1-6. Rev Argent Microbiol. 1979. PMID: 538236 Spanish. No abstract available.
-
Engineering of therapeutic antibodies to minimize immunogenicity and optimize function.Adv Drug Deliv Rev. 2006 Aug 7;58(5-6):640-56. doi: 10.1016/j.addr.2006.01.026. Epub 2006 May 23. Adv Drug Deliv Rev. 2006. PMID: 16904789 Review.
-
Antigen-antibody reactions.Immunol Ser. 1990;50:123-41. Immunol Ser. 1990. PMID: 2091754 Review. No abstract available.
Cited by
-
Interaction of purified precipitating and non-precipitating (coprecipitating) antibodies with hapten and with haptenated protein. Evidence of an asymmetric antibody molecule.Immunology. 1984 Jul;52(3):449-56. Immunology. 1984. PMID: 6745995 Free PMC article.
-
Immunoglobulin G Expression in Human Sperm and Possible Functional Significance.Sci Rep. 2016 Feb 1;6:20166. doi: 10.1038/srep20166. Sci Rep. 2016. PMID: 26833114 Free PMC article.
-
Structure of asymmetric non-precipitating antibody: presence of a carbohydrate residue in only one Fab region of the molecule.Immunology. 1986 Feb;57(2):311-7. Immunology. 1986. PMID: 3081439 Free PMC article.
-
A study of the possible role of Fab-glycosylated IgG in tumor immunity.Cancer Immunol Immunother. 2021 Jul;70(7):1841-1851. doi: 10.1007/s00262-020-02809-z. Epub 2021 Jan 3. Cancer Immunol Immunother. 2021. PMID: 33388997 Free PMC article.
-
A New Classification System for IgG4 Autoantibodies.Front Immunol. 2018 Feb 12;9:97. doi: 10.3389/fimmu.2018.00097. eCollection 2018. Front Immunol. 2018. PMID: 29483905 Free PMC article. Review.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources