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. 1980 Dec;36(3):837-46.
doi: 10.1128/JVI.36.3.837-846.1980.

Nucleic acids of respiratory syncytial virus

D M Lambert, M W Pons, G N Mbuy, K Dorsch-Hasler

Nucleic acids of respiratory syncytial virus

D M Lambert et al. J Virol. 1980 Dec.

Abstract

Analysis of purified respiratory syncytial virus revealed that the virion RNA was composed of 50S, 28S, 18S, and 4S species. The 18S and 28S species were presumed to represent host rRNA since virus grown in actinomycin D-treated cells contained only 50S and 4S RNAs. Actinomycin D treatment stimulated production of infectious respiratory syncytial virus 5- to 10-fold. The 50S virion RNA was shown to hybridize with polyadenylated mRNA's isolated from infected cells, indicating that respiratory syncytial virus RNA is of negative-strand sense. Six mRNA's were identified by polyacrylamide gel electrophoresis.

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