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. 1980 Nov 28;72(3):175-80.
doi: 10.1007/BF00572661.

Screening Fusarium strains isolated from overwintered Canadian grains for trichothecenes

P M Scott, J Harwig, B J Blanchfield

Screening Fusarium strains isolated from overwintered Canadian grains for trichothecenes

P M Scott et al. Mycopathologia. .

Abstract

A survey of 38 samples of Canadian overwintered grains showed that 14 (37%) contained viable Fusarium. Of a total of 38 Fusarium isolates, cultured on autoclaved corn, 20 (from 7 grain samples) showed toxicity to brine shrimp larvae and 12 (from 5 samples) produced levels of trichothecenes detectable by thin layer chromatography. The principal trichothecene found was T-2 toxin, produced by 10 strains and accompanied in half of these by neosolaniol; some of these strains were identified as F. sporotrichioides Sherbakoff. Two strains of F. poae (Peck) Wollenw. formed small amounts of diacetoxyscirpenol. T-2 toxin was the most toxic of 8 trichothecenes tested on brine shrimp larvae; the wide range of toxicities limits the usefulness of this bioassay as a general screening method for trichothecenes.

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