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. 1980 Nov 25;8(22):5193-205.
doi: 10.1093/nar/8.22.5193.

Solid phase phosphotriester synthesis of large oligodeoxyribonucleotides on a polyamide support

A F Markham, M D Edge, T C Atkinson, A R Greene, G R Heathcliffe, C R Newton, D Scanlon
Free PMC article

Solid phase phosphotriester synthesis of large oligodeoxyribonucleotides on a polyamide support

A F Markham et al. Nucleic Acids Res. .
Free PMC article

Abstract

Phosphotriester solid phase methodology on a polyamide support [(1980) Nucleic Acids Research, 8, 1081-1096] has been extended for the rapid synthesis of the tetradecanucleotide, d(AGTTGTTTGTAGTT), the octadecanucleotide, d(GTGGGTTTGGGGCAGGTC), and the heneicosanucleotide, d(GTGCTCTTATCCTCTTGGCTC). Thus, oligodeoxyribonucleotides comparable in size to those obtained by solution synthesis are readily accessible using solid phase techniques. An approach to the purification of the synthetic octadecanucleotide without recourse to high performance liquid chromatography is described.

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References

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