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. 1980 Dec 11;8(23):5703-14.
doi: 10.1093/nar/8.23.5703.

Purification and partial characterization of a DNA polymerase alpha species from calf thymus

F Grosse, G Krauss
Free PMC article

Purification and partial characterization of a DNA polymerase alpha species from calf thymus

F Grosse et al. Nucleic Acids Res. .
Free PMC article

Abstract

We have purified a DNA polymerase alpha species from calf thymus to near homogeneity. The enzyme sediments at 5.7 S and contains two polypeptides of 123000 and 134000 daltons in about equimolar ratio. The enzyme is inhibited by aphidicolin and N-ethylmaleimide, and retains its activity in buffers containing moderate salt conditions. Activated DNA is a better substrate than poly-(dA) . (dT) 10.

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References

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