doi: 10.1093/nar/8.23.5703.
Purification and partial characterization of a DNA polymerase alpha species from calf thymus
- PMID: 7465425
- PMCID: PMC324335
- DOI: 10.1093/nar/8.23.5703
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Purification and partial characterization of a DNA polymerase alpha species from calf thymus
Nucleic Acids Res.
.
Free PMC article
Abstract
We have purified a DNA polymerase alpha species from calf thymus to near homogeneity. The enzyme sediments at 5.7 S and contains two polypeptides of 123000 and 134000 daltons in about equimolar ratio. The enzyme is inhibited by aphidicolin and N-ethylmaleimide, and retains its activity in buffers containing moderate salt conditions. Activated DNA is a better substrate than poly-(dA) . (dT) 10.
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