Characterization of IS1221 from Mycoplasma hyorhinis: expression of its putative transposase in Escherichia coli incorporates a ribosomal frameshift mechanism
- PMID: 7476162
- DOI: 10.1111/j.1365-2958.1995.tb02429.x
Characterization of IS1221 from Mycoplasma hyorhinis: expression of its putative transposase in Escherichia coli incorporates a ribosomal frameshift mechanism
Abstract
Seven complete and two partial copies of IS1221 variants from Mycoplasma hyorhinis and Mycoplasma hyopneumoniae characterized to date have established a consensus IS1221 as a 1513 bp element with unique structural characteristics resembling the IS3 family of bacterial insertion sequences. Each IS1221 copy contains highly conserved 28 bp imperfect terminal inverted repeats and three distinctive internal inverted repeats (LIR, RIR and IIR). IIR is located within the coding region of the element and it is proposed that it plays a critical role in the regulation of putative transposase expression. Consensus IS1221 and one particular copy, G1135.2, contain a single long open reading frame (ORF). Two potential initiation codons are present at nucleotide 46 (AUG46) and nucleotide 397 (AUG397) and both are preceded by strong ribosome-binding sites. Both initiation codons can be used efficiently in an Escherichia coli T7 expression system. The LIR has a negative regulatory effect on translation initiation from AUG46. A-1 translational frameshift event is shown to be involved in expression of the IS1221 ORF and results in the production of 20 kDa and 6 kDa truncated proteins from the respective upstream initiation codons of the IS1221 ORF. Base substitution and deletion mutations in sequences resembling characterized motifs in documented examples of translational frameshifting resulted in a significant increase in the full-length products and a corresponding decrease in the truncated products from the IS1221 ORF. In contrast to the usual -1 frameshift regulatory event in the IS3 family, which produces a transframe fusion product as the active transposase, IS1221 may have evolved a high-frequency -1 frameshift mechanism that produces a truncated product from the upstream coding domain and thereby results in the regulated low-level production of the full-length presumptive transposase.
Similar articles
-
Translational control in production of transposase and in transposition of insertion sequence IS3.J Mol Biol. 1994 Feb 4;235(5):1406-20. doi: 10.1006/jmbi.1994.1097. J Mol Biol. 1994. PMID: 8107082
-
The insertion sequence element ISRm2011-2 belongs to the IS630-Tc1 family of transposable elements and is abundant in Rhizobium meliloti.Gene. 1995 Sep 22;163(1):59-64. doi: 10.1016/0378-1119(95)00371-c. Gene. 1995. PMID: 7557479
-
IS1630 of Mycoplasma fermentans, a novel IS30-type insertion element that targets and duplicates inverted repeats of variable length and sequence during insertion.J Bacteriol. 1999 Dec;181(24):7597-607. doi: 10.1128/JB.181.24.7597-7607.1999. J Bacteriol. 1999. PMID: 10601219 Free PMC article.
-
IS1-encoded proteins, InsA and the InsA-B'-InsB transframe protein (transposase): functions deduced from their DNA-binding ability.Adv Biophys. 1995;31:209-22. doi: 10.1016/0065-227x(95)99393-4. Adv Biophys. 1995. PMID: 7625275 Review.
-
Rhs elements of Escherichia coli: a family of genetic composites each encoding a large mosaic protein.Mol Microbiol. 1994 Jun;12(6):865-71. doi: 10.1111/j.1365-2958.1994.tb01074.x. Mol Microbiol. 1994. PMID: 7934896 Review.
Cited by
-
Fluorescent T7 display phages obtained by translational frameshift.Nucleic Acids Res. 2006;34(20):e137. doi: 10.1093/nar/gkl600. Epub 2006 Oct 13. Nucleic Acids Res. 2006. PMID: 17040895 Free PMC article.
-
Genome sequence of Mycoplasma hyorhinis strain GDL-1.J Bacteriol. 2012 Apr;194(7):1848. doi: 10.1128/JB.00033-12. J Bacteriol. 2012. PMID: 22408248 Free PMC article.
-
Characterization of Halomonas sp. ZM3 isolated from the Zelazny Most post-flotation waste reservoir, with a special focus on its mobile DNA.BMC Microbiol. 2013 Mar 14;13:59. doi: 10.1186/1471-2180-13-59. BMC Microbiol. 2013. PMID: 23497212 Free PMC article.
-
Molecular biology and pathogenicity of mycoplasmas.Microbiol Mol Biol Rev. 1998 Dec;62(4):1094-156. doi: 10.1128/MMBR.62.4.1094-1156.1998. Microbiol Mol Biol Rev. 1998. PMID: 9841667 Free PMC article. Review.
-
Identification and distribution of insertion sequences of Paracoccus solventivorans.Appl Environ Microbiol. 2003 Dec;69(12):7002-8. doi: 10.1128/AEM.69.12.7002-7008.2003. Appl Environ Microbiol. 2003. PMID: 14660342 Free PMC article.
Publication types
MeSH terms
Substances
Associated data
- Actions
Grants and funding
LinkOut - more resources
Full Text Sources