Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 1995 Apr-Jun:(2):21-6.

[Polymerase chain reaction and ligase reactions. Principles, traditional methods, and innovations]

[Article in Russian]
A S Belokhvostov
  • PMID: 7477029
Review

[Polymerase chain reaction and ligase reactions. Principles, traditional methods, and innovations]

[Article in Russian]
A S Belokhvostov. Mol Gen Mikrobiol Virusol. 1995 Apr-Jun.

Abstract

The polymerase chain reaction (PCR) is a favorite among the diagnostic DNA probes. Besides the traditional PCR scheme, the author considers the new strictly quantitative modifications representing a combination of PCR with semiautomated immunoassay, this simplifying the practical use of the method. Errors most frequently occurring in PCR are discussed. The ligase chain reaction, more rapid than PCR, and permitting one to obtain a billion of DNA molecule copies in 30 min, is described, and prospects of its use for rapid diagnosis shown.

PubMed Disclaimer