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. 1995 Oct 11;23(19):3944-8.
doi: 10.1093/nar/23.19.3944.

Mutation detection using immobilized mismatch binding protein (MutS)

R Wagner  1 , P Debbie, M Radman
Affiliations
Free PMC article

Mutation detection using immobilized mismatch binding protein (MutS)

R Wagner et al. Nucleic Acids Res. .
Free PMC article

Abstract

An accurate and highly sensitive mutation detection assay has been developed. The assay is based on the detection of mispaired and unpaired bases by immobilized mismatch binding protein (Escherichia coli MutS). The assay can detect most mismatches and all single base substitution mutations, as well as small addition or deletion mutations. The assay is simple to use and does not require the use of either radioactivity or gel electrophoresis.

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