Fluorescence polarization assay for endothelin-converting enzymes
- PMID: 7479323
- DOI: 10.1016/0196-9781(95)00032-f
Fluorescence polarization assay for endothelin-converting enzymes
Abstract
The conversion of big endothelin to endothelin by alpha-chymotrypsin was determined by following its single Trp fluorescence polarization. This provides a novel, simple, fast, and sensitive identifying assay in the search for a native endothelin-converting enzyme.
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