Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1995 Jul;113(1):59-67.
doi: 10.1016/s0021-9975(05)80069-6.

An immunohistochemical investigation of porcine epidemic diarrhoea

M Sueyoshi  1 , T Tsuda, K Yamazaki, K Yoshida, M Nakazawa, K Sato, T Minami, K Iwashita, M Watanabe, Y Suzuki, et al.
Affiliations

An immunohistochemical investigation of porcine epidemic diarrhoea

M Sueyoshi et al. J Comp Pathol. 1995 Jul.

Abstract

A sudden outbreak of epidemic diarrhoea of piglets occurred in Japan, the principal features being watery diarrhoea, dehydration and high mortality in newborn animals. The microscopical lesions were villous atrophy in the small intestine, the villous enterocytes being vacuolated and cuboidal in shape. The villus-crypt ratio was severely reduced, varying from 1:1 to 3:1. Transmission electron microscopy showed numerous coronaviruses within the cytoplasm of enterocytes and among microvilli. Specific antigens of porcine epidemic diarrhoea (PED) virus were detected in the cytoplasm of enterocytes by the streptavidin-biotin (SAB) technique. Infected cells, which were most abundant in the villous epithelia of the jejunum and ileum, were present in small numbers in the large intestine, the crypt epithelia, the lamina propria and Peyer's patches. The study suggests that the SAB technique is useful for the diagnosis of PED.

PubMed Disclaimer

References

    1. Chasey D., Cartwright S.F. Virus-like particles associated with porcine epidemic diarrhoea. Research in Veterinary Science. 1978;25:255–256. - PMC - PubMed
    1. Cox E., Hooyberghs J., Pensaert M.B. Sites of replication of a porcine respiratory coronavirus related to transmissible gastroenteritis virus. Research in Veterinary Science. 1990;48:165–169. - PMC - PubMed
    1. Debouck P., Pensaert M. Experimental infection of pigs with a new porcine enteric coronavirus CV 777. American Journal of Veterinary Research. 1980;41:219–223. - PubMed
    1. Debouck P., Pensaert M., Coussement W. The pathogenesis of an enteric infection in pigs, experimentally induced by the coronavirus-like agent, CV 777. Veterinary Microbiology. 1981;6:157–165.
    1. Ducatelle R., Coussement W., Charlier G., Debouck P., Hoorens J. Three-dimensional sequential study of the intestinal surface in experimental porcine CV 777 coronavirus enteritis. Zentralblatt für Veterinärmedizin, B. 1981;28:483–493. - PMC - PubMed

MeSH terms