Function-dependent anatomical parameters of rabbit masseter motor units

Abstract

Rabbit masseter motor units (22) were studied by stimulation of trigeminal motoneurons. We tested the hypotheses that masseter motor units facilitate fine motor control by concentrating fibers in small areas and that the distribution of motor unit fibers depends on the fiber type. The twitch contraction time and the isometric tetanic force were registered. The motor unit fibers were depleted of their glycogen by prolonged stimulation. Serial sections of the entire muscle were stained with the periodic acid Schiff (PAS) and monoclonal antibody stains. The muscle fibers of the motor unit were mapped and identified by four myosin heavy-chain (MHC) isoforms: I, IIA, IID, and cardiac-alpha. In the PAS-stained sections, anatomical parameters of the motor units, affecting the force output, were analyzed: the innervation ratio (IR), motor unit territory area (TA), and relative (R-DENS) and absolute (A-DENS) motor unit fiber densities. The fiber cross-sectional area (F-CSA) was measured for each MHC fiber type. The F-CSA sum of all motor unit fibers, the physiological cross-sectional area (P-CSA), was calculated. The IR ranged between 77 and 720 fibers (mean, 267). The mean TA was 8.71 mm2 (range, 4.45 to 19.58). The mean R-DENS was 10 fibers per 100; the A-DENS was 31 fibers per mm2. Linear correlations were found between the IR and the R-DENS and between the tetanic force and the IR. The F-CSAs showed a stepwise increase in value from type I- to IID-MHC fibers. The mean P-CSA was 0.90 mm2 (range, 0.09 to 2.97). A high linear correlation was noted between the P-CSA and the tetanic force. In conclusion, increase of motor unit size expressed in higher fiber counts and forces is accomplished by increase of the fiber density. Thus, forces can be exerted selectively in restricted regions of the masseter muscle. Differences in fiber orientation due to complex muscle pinnation emphasize the possibility of an accurate muscle performance.