Rapid purification of recombinant Taq DNA polymerase by freezing and high temperature thawing of bacterial expression cultures
- PMID: 7501478
- PMCID: PMC307412
- DOI: 10.1093/nar/23.21.4518
Rapid purification of recombinant Taq DNA polymerase by freezing and high temperature thawing of bacterial expression cultures
Similar articles
-
Rapid purification of high-activity Taq DNA polymerase.Nucleic Acids Res. 1993 Oct 11;21(20):4850-1. doi: 10.1093/nar/21.20.4850. Nucleic Acids Res. 1993. PMID: 8233838 Free PMC article.
-
Purification of Thermus aquaticus DNA polymerase expressed in Escherichia coli.Anal Biochem. 1990 Dec;191(2):396-400. doi: 10.1016/0003-2697(90)90238-5. Anal Biochem. 1990. PMID: 2085185
-
Single-step purification of recombinant Thermus aquaticus DNA polymerase using DNA-aptamer immobilized novel affinity magnetic beads.Biotechnol Prog. 2007 Jan-Feb;23(1):146-54. doi: 10.1021/bp0602505. Biotechnol Prog. 2007. PMID: 17269682
-
Purification of viral polymerases: general considerations.Methods Enzymol. 1996;275:3-16. doi: 10.1016/s0076-6879(96)75003-6. Methods Enzymol. 1996. PMID: 9026645 Free PMC article. Review. No abstract available.
-
The polymerase chain reaction.Genet Eng (N Y). 1990;12:115-37. doi: 10.1007/978-1-4613-0641-2_8. Genet Eng (N Y). 1990. PMID: 1369998 Review. No abstract available.
Cited by
-
In vitro evolution of thermostable p53 variants.Protein Sci. 1999 Apr;8(4):731-40. doi: 10.1110/ps.8.4.731. Protein Sci. 1999. PMID: 10211819 Free PMC article.
-
Directed evolution of aminoglycoside phosphotransferase (3') type IIIa variants that inactivate amikacin but impose significant fitness costs.PLoS One. 2013 Oct 18;8(10):e76687. doi: 10.1371/journal.pone.0076687. eCollection 2013. PLoS One. 2013. PMID: 24204655 Free PMC article.
-
Substrate ambiguous enzymes within the Escherichia coli proteome offer different evolutionary solutions to the same problem.Mol Biol Evol. 2013 Sep;30(9):2001-12. doi: 10.1093/molbev/mst105. Epub 2013 May 31. Mol Biol Evol. 2013. PMID: 23728795 Free PMC article.
-
DNA polymerase active site is highly mutable: evolutionary consequences.Proc Natl Acad Sci U S A. 2000 May 9;97(10):5095-100. doi: 10.1073/pnas.97.10.5095. Proc Natl Acad Sci U S A. 2000. PMID: 10805772 Free PMC article.
-
Do-it-yourself biology: challenges and promises for an open science and technology movement.Syst Synth Biol. 2013 Sep;7(3):115-26. doi: 10.1007/s11693-013-9116-4. Epub 2013 Aug 2. Syst Synth Biol. 2013. PMID: 24432149 Free PMC article.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources