Standardization of HeLa cells preparations as the source of snRNA's for preliminary study on the effectors of pre-mRNA splicing
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- PMID: 7503638
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Standardization of HeLa cells preparations as the source of snRNA's for preliminary study on the effectors of pre-mRNA splicing
Arch Immunol Ther Exp (Warsz).
1994.
Abstract
Physical, chemical and biological parameters of HeLa cell cultures, which ensure obtaining standard nuclear material to be used as a source of snRNP and snRNA, have been determined. The secondary structure of U1 snRNA as the result of the conformational studies performed using S1 nuclease, A and T1 ribonuclease and Pb(II) ions, and reproducibility of the results obtained after 6-48 h exposure of the cells to actinomycin D at high concentration permit to accept the proposed method of standardization as satisfactory for investigation into snRNP and snRNA.
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