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. 1995 Dec 14;217(2):407-11.
doi: 10.1006/bbrc.1995.2791.

Characterization of a specific monoclonal antibody 9F5-3a and the development of assay system for oxidized HDL

T Nakajima  1 , Y Sakagishi, T Katahira, A Nagata, T Kuwae, H Nakamura, I Inoue, K Takahashi, S Katayama, T Komoda
Affiliations

Characterization of a specific monoclonal antibody 9F5-3a and the development of assay system for oxidized HDL

T Nakajima et al. Biochem Biophys Res Commun. .

Abstract

We obtained a monoclonal antibody 9F5-3a against oxidative low-density lipoprotein (LDL) modified with CuSO4 and established a sandwich ELISA for detection of oxidized high-density lipoprotein (oxHDL). The 9F5-3a was reacted strongly with oxHDL and to a lesser degree with oxLDL and LDL. In contrast, little or no reactivity was found with HDL. When the generation of oxHDL was limited by the addition of alpha-tocopherol and catalase, reactivity to 9F5-3a was reduced. Incubation of oxHDL with excess lyso-phosphatidylcholine (lyso-PC) also reduced immunoreactivity, but not by only lyso-PC. These results suggested that the epitope is possibly associated with oxHDL-linked lyso-PC induced mainly by the hydroxyl radical.

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