Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1993 Dec;143(6):1579-85.

Melanocyte lineage-specific antigens recognized by monoclonal antibodies NKI-beteb, HMB-50, and HMB-45 are encoded by a single cDNA

G J Adema  1 , A J de Boer, R van 't Hullenaar, M Denijn, D J Ruiter, A M Vogel, C G Figdor
Affiliations

Melanocyte lineage-specific antigens recognized by monoclonal antibodies NKI-beteb, HMB-50, and HMB-45 are encoded by a single cDNA

G J Adema et al. Am J Pathol. 1993 Dec.

Abstract

The glycoproteins recognized by monoclonal antibody (MAb) NKI-beteb are among the best diagnostic markers for human melanoma. MAb NKI-beteb reacts with melanoma cells throughout tumor development and does not cross-react with other tumor or normal cells, except for cells of the melanocytic lineage. Two other melanocyte lineage-specific MAbs, HMB-50 and HMB-45, show a specificity and staining pattern strikingly similar to the ones observed for NKI-beteb. Herein, we demonstrate that all three MAbs recognize protein products encoded by a single cDNA. Expression of this cDNA in BLM cells results in immunoreactivity with all three MAbs. In addition, we demonstrate co-distribution of the RNA species detected by the cDNA with the proteins recognized by the MAbs in tissue sections.

PubMed Disclaimer

References

    1. Virology. 1973 Apr;52(2):456-67 - PubMed
    1. J Histochem Cytochem. 1992 Nov;40(11):1761-7 - PubMed
    1. Biochemistry. 1979 Nov 27;18(24):5294-9 - PubMed
    1. Proc Natl Acad Sci U S A. 1982 Mar;79(6):2018-22 - PubMed
    1. J Cancer Res Clin Oncol. 1984;108(2):197-203 - PubMed

Publication types

MeSH terms