Immune recognition of linear antigenic regions within the hepatitis B pre-C and C-gene translation products using synthetic peptides

Abstract

The antibody recognition of linear regions within the amino acid (aa) sequence of hepatitis B (HB) core antigen (HBcAg), e antigen (HBeAg), and pre-C region was investigated in 46 patients infected with hepatitis B virus (HBV), and one immunized rabbit. Peptide analogues were synthesized to cover the complete product of the C-gene, including the pre-C region using various synthetic methods. Two carriers of hepatitis B surface antigen (HBsAg) with anti-HBe, recognized pin-bound decapeptides covering amino acid (aa) 76-83 of HBc/eAg, and the most essential residues were found to be Asp78, Pro79, Arg82, and Asp83. Pre-C peptides were recognized by IgG1 or IgG3 in sera from two out of ten cases with acute HB, in four out of twelve sera from HBeAg-positive carriers of HBsAg, and in two out of twelve sera from anti-HBe-positive carriers of HBsAg. Two sera from the cases of acute HB showed strong reactivity of the IgG3 isotype with HBc/eAg peptides 61-85. Five of the sera from HBeAg-positive carriers of HBsAg were weakly reactive with peptides 41-60, 61-85, 121-140, and/or 141-160. Eight of the sera from anti-HBe-positive carriers of HBsAg recognized aa 121-140 of HBc/e with IgG1, IgG3, and/or IgG4 isotypes. IgG from one immunized rabbit recognized peptides 1-20, 61-85, and 71-90, and the T-cells recognized peptides 1-20 and 71-90. Thus, human and rabbit antibodies recognize linear antigenic regions within the pre-C, and within regions 1-20, 41-60, 61-85, 121-140, and 141-160 of HBcAg.(ABSTRACT TRUNCATED AT 250 WORDS)