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. 1993 Dec;25(12):854-6.

Microwave-assisted staining of mucosal mast cells and granulated intra-epithelial lymphocytes after formalin fixation

Affiliations
  • PMID: 7509328

Microwave-assisted staining of mucosal mast cells and granulated intra-epithelial lymphocytes after formalin fixation

D Biernacka et al. Histochem J. 1993 Dec.

Abstract

Rat jejunum was fixed with either formalin or methanol-formalin acetic acid (MFAA) and stained with Astra Blue or Alcian Blue with or without microwave irradiation. Staining of both mucosal mast cells and granulated intra-epithelial lymphocytes after formalin fixation was considerably improved by microwave irradiation. On the other hand, microwave irradiation slightly impaired staining of mucosal mast cells (MMC) and even more strongly granulated intra-epithelial lymphocytes (GIEL) after MFAA fixation.

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