Effect of progesterone on galanin mRNA levels in the hypothalamus and the pituitary: correlation with the gonadotropin surge
- PMID: 7509463
- DOI: 10.1159/000126587
Effect of progesterone on galanin mRNA levels in the hypothalamus and the pituitary: correlation with the gonadotropin surge
Abstract
Galanin has been shown to be an important neuropeptide associated with the preovulatory surge of gonadotropins. Since the preovulatory surge of gonadotropins is induced by the action of estrogens and progesterone, the present study examined the effect of estrogens and progesterone on hypothalamic and pituitary galanin mRNA levels. Galanin mRNA was present as the 900-base pair message in the hypothalamus and the pituitary. In the 27-day-old ovariectomized immature rat, treatment with 2 micrograms of estradiol for 2 days did not bring about any changes in hypothalamic galanin mRNA levels. In adult rats, ovariectomy also did not bring about any changes in hypothalamic galanin mRNA levels. However, 14 days of estradiol replacement increased hypothalamic galanin mRNA levels. Progesterone treatment in the estrogen-primed ovariectomized immature rat resulted in a significant increase in hypothalamic galanin mRNA levels at 12:00 and 14:00 h. This increase occurred at the time of the initiation of the progesterone-induced LH and FSH surges. Estrogen treatment enhanced pituitary galanin mRNA levels which were further increased by progesterone treatment at 12:00 h, the time of the initiation of the gonadotropin surge by progesterone. In the PMSG-primed immature rat, pituitary galanin mRNA levels were increased due to ovarian estrogen and progesterone secretion as a result of PMSG treatment. Pituitary galanin mRNA levels were decreased by the anti-progestin RU486 at 12:00 and 20:00 h, times that coincided with a decrease in serum LH by RU486 as compared to the PMSG-treated controls.(ABSTRACT TRUNCATED AT 250 WORDS)
Similar articles
-
Regulation of anterior pituitary gonadotropin subunit mRNA levels during the preovulatory gonadotropin surge: a physiological role of progesterone in regulating LH-beta and FSH-beta mRNA levels.J Steroid Biochem Mol Biol. 1993 Oct;46(4):427-37. doi: 10.1016/0960-0760(93)90097-g. J Steroid Biochem Mol Biol. 1993. PMID: 8217874
-
Activation-dependent regulation of galanin gene expression in gonadotropin-releasing hormone neurons in the female rat.Endocrinology. 1994 May;134(5):1991-8. doi: 10.1210/endo.134.5.7512492. Endocrinology. 1994. PMID: 7512492
-
Interaction between ovarian and adrenal steroids in the regulation of gonadotropin secretion.J Steroid Biochem Mol Biol. 1992 Mar;41(3-8):495-513. doi: 10.1016/0960-0760(92)90375-s. J Steroid Biochem Mol Biol. 1992. PMID: 1562521 Review.
-
Evidence that progesterone modulates anterior pituitary neuropeptide Y levels during the progesterone-induced gonadotropin surge in the estrogen-primed intact immature female rat.J Steroid Biochem Mol Biol. 1995 May;52(5):497-504. doi: 10.1016/0960-0760(95)00011-n. J Steroid Biochem Mol Biol. 1995. PMID: 7748815
-
Integration of the effects of estradiol and progesterone in the modulation of gonadotropin secretion.J Steroid Biochem. 1987;27(4-6):665-75. doi: 10.1016/0022-4731(87)90135-x. J Steroid Biochem. 1987. PMID: 2826901 Review.
Cited by
-
Puberty onset in female rats: relationship with fat intake, ovarian steroids and the peptides, galanin and enkephalin, in the paraventricular and medial preoptic nuclei.J Neuroendocrinol. 2009 Jun;21(6):538-49. doi: 10.1111/j.1365-2826.2009.01870.x. J Neuroendocrinol. 2009. PMID: 19500224 Free PMC article.
-
Hirsutism, virilism, polycystic ovarian disease, and the steroid-gonadotropin-feedback system: a career retrospective.Am J Physiol Endocrinol Metab. 2012 Jan 1;302(1):E4-E18. doi: 10.1152/ajpendo.00488.2011. Epub 2011 Oct 25. Am J Physiol Endocrinol Metab. 2012. PMID: 22028409 Free PMC article. Review.
-
Gender-specific association of galanin polymorphisms with HPA-axis dysregulation, symptom severity, and antidepressant treatment response.Neuropsychopharmacology. 2010 Jun;35(7):1583-92. doi: 10.1038/npp.2010.30. Epub 2010 Mar 17. Neuropsychopharmacology. 2010. PMID: 20237460 Free PMC article.
-
Increased intake of ethanol and dietary fat in galanin overexpressing mice.Alcohol. 2009 Dec;43(8):571-80. doi: 10.1016/j.alcohol.2009.09.025. Alcohol. 2009. PMID: 20004335 Free PMC article.
-
Insights into Potential Targets for Therapeutic Intervention in Epilepsy.Int J Mol Sci. 2020 Nov 13;21(22):8573. doi: 10.3390/ijms21228573. Int J Mol Sci. 2020. PMID: 33202963 Free PMC article. Review.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources