Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1994 Jan;60(1):348-52.
doi: 10.1128/aem.60.1.348-352.1994.

Transcriptional enhancement of the Listeria monocytogenes PCR and simple immunoenzymatic assay of the product using anti-RNA:DNA antibodies

B W Blais  1
Affiliations
Comparative Study

Transcriptional enhancement of the Listeria monocytogenes PCR and simple immunoenzymatic assay of the product using anti-RNA:DNA antibodies

B W Blais. Appl Environ Microbiol. 1994 Jan.

Abstract

A method was developed to enhance the sensitivity of a Listeria monocytogenes PCR detection system by in vitro transcription of amplicons incorporating bacteriophage T7 RNA polymerase promoter sequences in one of the priming oligonucleotides. The resulting transcript can be detected by hybridization with a DNA probe immobilized in the wells of a microtiter plate, followed by immunoenzymatic assay of the RNA-DNA hybrids with an anti-RNA-DNA hybrid antibody. This highly sensitive method was reactive in the assay of various L. monocytogenes isolates but not with other Listeria or non-Listeria species.

PubMed Disclaimer

References

    1. Methods Enzymol. 1980;70(A):70-85 - PubMed
    1. Appl Environ Microbiol. 1993 Sep;59(9):2795-800 - PubMed
    1. Science. 1988 Jan 29;239(4839):487-91 - PubMed
    1. Infect Immun. 1988 Apr;56(4):766-72 - PubMed
    1. DNA. 1988 May;7(4):287-95 - PubMed

Publication types

MeSH terms

LinkOut - more resources