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. 1994 Mar 18;263(5153):1612-5.
doi: 10.1126/science.7510419.

Requirement for transcription factor IRF-1 in NO synthase induction in macrophages

R Kamijo  1 , H Harada, T Matsuyama, M Bosland, J Gerecitano, D Shapiro, J Le, S I Koh, T Kimura, S J Green, et al.
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Requirement for transcription factor IRF-1 in NO synthase induction in macrophages

R Kamijo et al. Science. .

Abstract

Production of nitric oxide (NO) by macrophages is important for the killing of intracellular infectious agents. Interferon (IFN)-gamma and lipopolysaccharide stimulate NO production by transcriptionally up-regulating the inducible NO synthase (iNOS). Macrophages from mice with a targeted disruption of the IFN regulatory factor-1 (IRF-1) gene (IRF-1-/- mice) produced little or no NO and synthesized barely detectable iNOS messenger RNA in response to stimulation. Two adjacent IRF-1 response elements were identified in the iNOS promoter. Infection with Mycobacterium bovis (BCG) was more severe in IRF-1-/- mice than in wild-type mice. Thus, IRF-1 is essential for iNOS activation in murine macrophages.

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