Modified technique for efficient detection of microsporidia
- PMID: 7517951
- PMCID: PMC267187
- DOI: 10.1128/jcm.32.4.1074-1075.1994
Modified technique for efficient detection of microsporidia
Abstract
Changes in temperature (from room temperature to 50 degrees C) and staining time (from 90 to 10 min) were evaluated as a means of improving the detection of microsporidia from stool specimens. A blinded and independent comparison of 50 known positive matched-specimen pairs by three technologists resulted in consistently easier microscopic detection. The background is clearer, and spores stain more intensely. Staining time is reduced by 80 min.
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