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Clinical Trial
. 1994 Apr;32(4):1074-5.
doi: 10.1128/jcm.32.4.1074-1075.1994.

Modified technique for efficient detection of microsporidia

Affiliations
Clinical Trial

Modified technique for efficient detection of microsporidia

E Kokoskin et al. J Clin Microbiol. 1994 Apr.

Abstract

Changes in temperature (from room temperature to 50 degrees C) and staining time (from 90 to 10 min) were evaluated as a means of improving the detection of microsporidia from stool specimens. A blinded and independent comparison of 50 known positive matched-specimen pairs by three technologists resulted in consistently easier microscopic detection. The background is clearer, and spores stain more intensely. Staining time is reduced by 80 min.

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