Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1994 Mar;101(3):223-32.
doi: 10.1007/BF00269548.

Ultrastructural localization of epidermal growth factor (EGF)-receptor transcripts in the cell nucleus using pre-embedding in situ hybridization in combination with ultra-small gold probes and silver enhancement

O C Sibon  1 , B M Humbel, A De Graaf, A J Verkleij, F F Cremers
Affiliations

Ultrastructural localization of epidermal growth factor (EGF)-receptor transcripts in the cell nucleus using pre-embedding in situ hybridization in combination with ultra-small gold probes and silver enhancement

O C Sibon et al. Histochemistry. 1994 Mar.

Abstract

A high-resolution in situ hybridization method is described for localizing epidermal growth factor (EGF)-receptor transcripts in nuclei of A431 epidermoid carcinoma cells. The method is based upon the use of ultra-small gold particles in combination with silver enhancement. The RNA of the EGF-receptor was detected mainly around the nucleoli. After removal of the DNA using nucleases and high salt extraction, the RNA of the EGF-receptor appears to be associated with the nuclear matrix. The RNA of the EGF-receptor was observed in close contact with the SC-35 splicing protein, but no exact colocalization was observed. These results demonstrate that high resolution pre-embedding in situ hybridization in combination with immunocytochemistry, both using ultra-small gold as a detection method, provides a powerful tool to unravel the organization of nuclear processes.

PubMed Disclaimer

References

    1. Cell Biol Int. 1993 Jan;17(1):1-11 - PubMed
    1. J Histochem Cytochem. 1991 Aug;39(8):1035-45 - PubMed
    1. Microsc Res Tech. 1993 May 1;25(1):2-11 - PubMed
    1. Exp Cell Res. 1991 Dec;197(2):319-22 - PubMed
    1. Cell Growth Differ. 1991 Mar;2(3):155-64 - PubMed

MeSH terms