Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1994 Jun;19(6):777-82.
doi: 10.1007/BF00967719.

Kainate binding to the AMPA receptor in rat brain

R A Hall  1 , M Kessler, G Lynch
Affiliations

Kainate binding to the AMPA receptor in rat brain

R A Hall et al. Neurochem Res. 1994 Jun.

Abstract

Displacement of [3H]AMPA and [3H]CNQX by kainate was measured in membranes and solubilized fractions from rat brain. In soluble fractions, plots of [3H]AMPA and [3H]CNQX binding displaced by kainate resulted in one-site fits with Ki values in the range of 1-3 microM. In membranes, plots of [3H]AMPA binding displaced by kainate resulted in graphs which were better fit by two-site regression analysis than by a one-site fit. The Ki value for the high-affinity component of these two-site fits was 3-9 microM and the low-affinity component Ki was in the range of 70-120 microM; similar values were determined for kainate displacement of [3H]CNQX. The presence of thiocyanate ions had no effect on kainate displacement of [3H]CNQX. Since the affinity for kainate of the presumed synaptic AMPA receptor is in the range of EC50 values for kainate determined from physiological studies, these data contribute further evidence for the idea that kainate binding to synaptic AMPA receptors may be responsible for many of kainate's physiological effects.

PubMed Disclaimer

References

    1. Nature. 1989 Dec 7;342(6250):643-8 - PubMed
    1. J Neurochem. 1982 Jan;38(1):173-8 - PubMed
    1. Brain Res. 1987 Feb 3;402(2):243-54 - PubMed
    1. Science. 1990 Aug 3;249(4968):556-60 - PubMed
    1. Nature. 1991 Jun 27;351(6329):745-8 - PubMed

Publication types

Substances