Interdependence of contractile responses of rat small mesenteric arteries on nitric oxide and cyclo-oxygenase and lipoxygenase products of arachidonic acid
- PMID: 7521254
- PMCID: PMC1910336
- DOI: 10.1111/j.1476-5381.1994.tb13080.x
Interdependence of contractile responses of rat small mesenteric arteries on nitric oxide and cyclo-oxygenase and lipoxygenase products of arachidonic acid
Abstract
1. We have examined the effects of nitric oxide inhibition, indomethacin and the dual lipoxygenase/cyclo-oxygenase inhibitor, 3-amino-1-[m-(trifluoromethyl)-phenyl]-2-pyrazoline (BW755C), on the responses of small mesenteric arteries of Wistar rats, with and without endothelium, to noradrenaline, potassium chloride, endothelin-1, acetylcholine and sodium nitroprusside. 2. Noradrenaline, potassium chloride and endothelin-1 caused concentration-dependent contraction of small mesenteric arteries. Indomethacin (14 microM) attenuated the contractile response to both noradrenaline and potassium chloride. The inhibitory action of indomethacin persisted in vessels treated with CHAPS. 3. Acetylcholine produced concentration-dependent relaxation in these vessels. Indomethacin (14 microM) had no significant effect on the acetylcholine concentration-response relationship. 4. NG-nitro-L-arginine methyl ester (L-NAME, 100 microM) potentiated the contractile response to both noradrenaline and potassium chloride and inhibited acetylcholine-induced relaxation. Indomethacin attenuated the effects of L-NAME. 5. BW755C inhibited the contractile response to noradrenaline and potassium chloride but not to endothelin-1. The inhibitory effects of BW755C persisted in the presence of indomethacin and in vessels treated with CHAPS. 6. BW755C enhanced endothelium-dependent relaxation, as assessed by the response to acetylcholine. In the presence of indomethacin, BW755C produced a shift to the right of the concentration-response curve to acetylcholine. 7. Inhibition of nitric oxide synthase with L-NAME, reversed the inhibitory effect of BW755C on noradrenaline- and potassium-induced contraction. L-NAME and BW755C in combination resulted in a shift to the right of the concentration-response curve to acetylcholine. 8. Sodium nitroprusside produced concentration-dependent relaxation of the vessels. Endothelium removal reduced the maximum relaxation to nitroprusside. BW755C did not alter the response to sodium nitroprusside in vessels with or without endothelium.9 .These data support the existence of two vasoconstrictor products of arachidonic acid released during contraction of small mesenteric arteries with noradrenaline and potassium chloride: a cyclo-oxygenase product and a lipoxygenase product both of which appear to be largely endothelium-independent.
Similar articles
-
Relative roles of nitric oxide and cyclo-oxygenase and lipoxygenase products of arachidonic acid in the contractile responses of rat renal arcuate arteries.Br J Pharmacol. 1994 Jun;112(2):369-76. doi: 10.1111/j.1476-5381.1994.tb13081.x. Br J Pharmacol. 1994. PMID: 8075854 Free PMC article.
-
Endothelium-dependent relaxation and noradrenaline sensitivity in mesenteric resistance arteries of streptozotocin-induced diabetic rats.Br J Pharmacol. 1992 Oct;107(2):393-9. doi: 10.1111/j.1476-5381.1992.tb12757.x. Br J Pharmacol. 1992. PMID: 1422588 Free PMC article.
-
Mesenteric arterial function in the rat in pregnancy: role of sympathetic and sensory-motor perivascular nerves, endothelium, smooth muscle, nitric oxide and prostaglandins.Br J Pharmacol. 1996 Apr;117(7):1463-70. doi: 10.1111/j.1476-5381.1996.tb15307.x. Br J Pharmacol. 1996. PMID: 8730740 Free PMC article.
-
Mechanisms of carotid body inhibition.Adv Exp Med Biol. 1994;360:229-35. doi: 10.1007/978-1-4615-2572-1_35. Adv Exp Med Biol. 1994. PMID: 7532901 Review. No abstract available.
-
How do fish oils affect vascular function?Clin Exp Pharmacol Physiol. 1995 Feb;22(2):71-81. doi: 10.1111/j.1440-1681.1995.tb01959.x. Clin Exp Pharmacol Physiol. 1995. PMID: 7621612 Review.
Cited by
-
Effect of tumour necrosis factor-alpha and interleukin 1beta on endothelium-dependent relaxation in rat mesenteric resistance arteries in vitro.Br J Pharmacol. 2003 Apr;138(7):1285-94. doi: 10.1038/sj.bjp.0705168. Br J Pharmacol. 2003. PMID: 12711629 Free PMC article.
-
Dual NEP/ECE inhibition improves endothelial function in mesenteric resistance arteries of 32-week-old SHR.Hypertens Res. 2017 Aug;40(8):738-745. doi: 10.1038/hr.2017.38. Epub 2017 Mar 16. Hypertens Res. 2017. PMID: 28298655
-
Increased constrictor tone induced by ouabain treatment in rats.J Cardiovasc Pharmacol. 2013 Aug;62(2):174-83. doi: 10.1097/FJC.0b013e3182955d33. J Cardiovasc Pharmacol. 2013. PMID: 23615157 Free PMC article.
-
Alterations of cyclo-oxygenase products and NO in responses to angiotensin II of resistance arteries from the spontaneously hypertensive rat.Br J Pharmacol. 1996 Dec;119(8):1635-41. doi: 10.1111/j.1476-5381.1996.tb16083.x. Br J Pharmacol. 1996. PMID: 8982512 Free PMC article.
-
Action of AT1 receptor antagonists on angiotensin II-induced tone in human isolated subcutaneous resistance arteries.Br J Pharmacol. 1999 Aug;127(8):1876-82. doi: 10.1038/sj.bjp.0702722. Br J Pharmacol. 1999. PMID: 10482919 Free PMC article.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources