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Comparative Study
. 1994 Jun;32(6):1492-6.
doi: 10.1128/jcm.32.6.1492-1496.1994.

Detection of Bartonella (Rochalimaea) quintana by routine acridine orange staining of broth blood cultures

A M Larson  1 , M J Dougherty, D J Nowowiejski, D F Welch, G M Matar, B Swaminathan, M B Coyle
Affiliations
Comparative Study

Detection of Bartonella (Rochalimaea) quintana by routine acridine orange staining of broth blood cultures

A M Larson et al. J Clin Microbiol. 1994 Jun.

Abstract

Bartonella quintana was isolated from 34 BACTEC nonradiometric aerobic resin blood cultures for 10 adults. Nine patients were initially diagnosed by routine acridine orange staining of routine cultures that had been incubated for 8 days. All subcultures grew on chocolate agar within 3 to 12 days (median, 6 days). The PLUS 26 high-volume aerobic resin medium, combined with acridine orange stain and subculture, is an effective system for detection and isolation of B. quintana from blood.

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