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. 1993 Fall;2(3):339-42.
doi: 10.1089/scd.1.1993.2.339.

Direct isolation and expansion of human CD34+ hematopoietic stem cells

J Lebkowski  1 , L Schain, M Harvey, A Moseley, T B Okarma
Affiliations

Direct isolation and expansion of human CD34+ hematopoietic stem cells

J Lebkowski et al. J Hematother. 1993 Fall.

Abstract

The isolation and expansion of CD34+ cells has numerous applications as supportive therapy for cytopenias that occur postchemotherapy. In this report, we describe a simple system to directly isolate and culture human CD34+ cells. Using this system, CD34+ cells isolated from bone marrow were cultured in the presence of GM-CSF, G-CSF, erythropoietin, stem cell factor, IL-1, IL-3, and IL-6. Cell expansions up to 100-fold were noted during the first 2 weeks of culture with high maintenance of CD34+ cells during the first week of culture. Maximal expansions of progenitors were observed at one week of culture, with, on average, 20-fold increases in CFU-GM number. The results support the feasibility of CD34+ cell expansion for clinical application.

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