Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1994 Sep 25;22(19):3866-70.
doi: 10.1093/nar/22.19.3866.

Matrix assisted laser desorption/ionization mass spectrometry of enzymatically synthesized RNA up to 150 kDa

F Kirpekar  1 , E Nordhoff, K Kristiansen, P Roepstorff, A Lezius, S Hahner, M Karas, F Hillenkamp
Affiliations
Free PMC article

Matrix assisted laser desorption/ionization mass spectrometry of enzymatically synthesized RNA up to 150 kDa

F Kirpekar et al. Nucleic Acids Res. .
Free PMC article

Abstract

Enzymatically synthesized RNA samples (in vitro transcripts) were analysed by matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS). Spectra of RNA up to 150 kDA (461 nucleotides) are shown. Polymerase generated sample heterogeneity and its contribution to mass resolution are discussed. A time course exonuclease digest of a 55 nt in vitro transcript was analyzed to investigate the performance of MALDI-MS on complex mixtures. Based on these data, the analysis by MALDI-MS of DNA sequencing reactions, produced by the action of an RNA polymerase, is discussed.

PubMed Disclaimer

References

    1. Nucleic Acids Res. 1993 Jul 25;21(15):3347-57 - PubMed
    1. Nucleic Acids Res. 1993 Jul 11;21(14):3191-6 - PubMed
    1. Rapid Commun Mass Spectrom. 1994 Feb;8(2):183-6 - PubMed
    1. J Am Chem Soc. 1970 Mar 25;92(6):1741-9 - PubMed
    1. Cell. 1983 Oct;34(3):865-79 - PubMed

Publication types