Characterization of putative small nuclear RNAs from Giardia lamblia

Abstract

Small nuclear RNAs (snRNAs), which have roles in RNA metabolism, have been found in all eukaryotic cells. Candidate snRNAs were identified in the primitive protozoan parasite, Giardia lamblia, by either immunoprecipitation of total RNA with antiserum directed against the 2,2,7-trimethylguanosine cap structure characteristic of snRNAs or by Northern hybridization with oligonucleotide probes complementary to snRNA consensus sequences. Isolated putative snRNAs include eight 2,2,7-trimethylguanosine-capped species: RNAs A through H, and one non-2,2,7-trimethylguanosine-capped species: RNA J. Single copy genes encoding RNA D, RNA H and RNA J were cloned and sequenced, and the 5' end of each RNA was determined by primer extension analysis. Certain characteristics suggest tentative identification of these Giardia RNAs as nucleolar RNAs with possible roles in rRNA processing.