[Nomographs for determination of sedimentation constants of nucleic acids by means of centrifugation in sucrose gradients using the "Sinco" ultracentrifuge]
- PMID: 752798
[Nomographs for determination of sedimentation constants of nucleic acids by means of centrifugation in sucrose gradients using the "Sinco" ultracentrifuge]
Abstract
A set of nomographs for determination of standard sedimentation constant values for DNA and RNA directly from the results of centrifugation without any marker is calculated and plotted for 9 Spinco bucket-rotors, 5 temperatures, and two commonly used sucrose gradients (5--20 and 15--30%).