Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1994 Dec 15;304 ( Pt 3)(Pt 3):683-6.
doi: 10.1042/bj3040683.

Expression of rat brain nitric oxide synthase in baculovirus-infected insect cells and characterization of the purified enzyme

C Harteneck  1 , P Klatt, K Schmidt, B Mayer
Affiliations

Expression of rat brain nitric oxide synthase in baculovirus-infected insect cells and characterization of the purified enzyme

C Harteneck et al. Biochem J. .

Abstract

Rat brain nitric oxide synthase was expressed to a high level in baculovirus-infected insect cells and purified to apparent homogeneity by affinity chromatography. The enzyme had a specific activity of approximately 1 mumol of citrulline.min-1.mg of protein-1 and contained 0.93, 0.45, 0.18 and 0.23 mol of haem, (6R)-5,6,7,8-tetrahydro-L-biopterin (H4biopterin), FAD and FMN per mol of subunit respectively.

PubMed Disclaimer

References

    1. Anal Biochem. 1976 May 7;72:248-54 - PubMed
    1. J Biol Chem. 1994 May 27;269(21):15147-53 - PubMed
    1. Arch Biochem Biophys. 1989 Nov 1;274(2):481-90 - PubMed
    1. Proc Natl Acad Sci U S A. 1990 Jan;87(2):682-5 - PubMed
    1. Nucleic Acids Res. 1990 Jul 11;18(13):4033 - PubMed

Publication types