[Isolation and characteristics of an ordered library of transcribed sequences of human chromosome 19 from hybrid human-hamster cells]
[Article in
Russian]
- PMID: 7530008
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[Isolation and characteristics of an ordered library of transcribed sequences of human chromosome 19 from hybrid human-hamster cells]
[Article in
Russian]
Bioorg Khim.
1994 Aug-Sep.
Abstract
A new technique based on Alu-PCR amplification of hn-RNA is described for the extraction of human-specific transcribed sequences from a hybrid cell line. Arrayed library of hn-cDNA was constructed and characterized by sequencing about 80 individual clones. A high enrichment by human-specific sequences (about 95%) was demonstrated.
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