doi: 10.1016/0896-6273(95)90279-1.
The jellyfish green fluorescent protein: a new tool for studying ion channel expression and function
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- PMID: 7531985
- DOI: 10.1016/0896-6273(95)90279-1
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The jellyfish green fluorescent protein: a new tool for studying ion channel expression and function
Neuron.
1995 Feb.
Free article
Abstract
Two methods are described for using the jellyfish green fluorescent protein (GFP) as a reporter gene for ion channel expression. GFP fluorescence can be used to identify the transfected cells, and to estimate the relative levels of ion channel expression, in cotransfection experiments. A GFP-NMDAR1 chimera can be constructed that produces a functional, fluorescent receptor subunit. These methods should facilitate studies of ion channel expression, localization, and processing.
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