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Comparative Study
. 1994 Apr;1(2):147-50.
doi: 10.1007/BF02921555.

Detection of proteins and sialoglycoproteins in polyacrylamide gels using eosin Y stain

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Comparative Study

Detection of proteins and sialoglycoproteins in polyacrylamide gels using eosin Y stain

F Lin et al. Mol Biotechnol. 1994 Apr.

Abstract

An eosin Y staining technique that permits detection of various proteins, including membrane sialoglycoproteins, in polyacrylamide gels is described. The sensitivity of the eosin Y staining method is comparable to silver staining. In addition, there is an added advantage of the antigenicity of the stained proteins being retained in a Western blot. Details of the procedure to obtain optimal staining results are described.

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