Detection of proteins and sialoglycoproteins in polyacrylamide gels using eosin Y stain
- PMID: 7532101
- DOI: 10.1007/BF02921555
Detection of proteins and sialoglycoproteins in polyacrylamide gels using eosin Y stain
Abstract
An eosin Y staining technique that permits detection of various proteins, including membrane sialoglycoproteins, in polyacrylamide gels is described. The sensitivity of the eosin Y staining method is comparable to silver staining. In addition, there is an added advantage of the antigenicity of the stained proteins being retained in a Western blot. Details of the procedure to obtain optimal staining results are described.