Characterization of a gene encoding a cysteine-rich keratin associated protein synthesized late in rabbit hair follicle differentiation

Abstract

Many different cysteine-rich proteins are synthesized during hair follicle differentiation, forming part of the interstitial matrix between bundles of intermediate filaments. We have isolated a rabbit gene (rKAP4L), a member of a multigene family that encodes a small cysteine-rich hair keratin associated protein. This is the first complete gene sequence for this family. The rKAP4L gene is expressed in the cortex of rabbit pelage hair follicles at a late stage of hair follicle differentiation, well after the synthesis of the other major hair proteins, the intermediate filament and glycine/tyrosine-rich keratin associated proteins, has commenced. The protein contains 36 mol % cysteine, with a molecular size of 13593 daltons, and its sequence appears to be based on a pentapeptide repeat. It is predicted to adopt a folded conformation characterized by beta-turns interspersed with short stretches of beta-sheet or random coil.