Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1995 Jul;96(1):646-52.
doi: 10.1172/JCI118080.

Expression and localization of the cystic fibrosis transmembrane conductance regulator mRNA and its protein in rat brain

A E Mulberg  1 , L P Resta, E B Wiedner, S M Altschuler, D M Jefferson, D L Broussard
Affiliations

Expression and localization of the cystic fibrosis transmembrane conductance regulator mRNA and its protein in rat brain

A E Mulberg et al. J Clin Invest. 1995 Jul.

Abstract

In previous studies we have characterized the expression of the cystic fibrosis transmembrane conductance regulator (CFTR) protein in clathrin-coated vesicles derived from bovine brain and in neurons of rat brain. In this study we have further characterized the expression of the CFTR protein mRNA and protein in rat brain with reverse transcriptase polymerase chain reaction amplification (RT-PCR), in situ hybridization, and immunocytochemistry. The expression of CFTR mRNA and protein in discrete areas of brain, including the hypothalamus, thalamus, and amygdaloid nuclei, which are involved in regulation of appetite and resting energy expenditure, is identical. The presence of CFTR in neurons localized to these regions of brain controlling homeostasis and energy expenditure may elucidate the pathogenesis of other nonpulmonary and gastrointestinal manifestations which commonly are observed in children with cystic fibrosis. Dysregulation of normal neuropeptide vesicle trafficking by mutant CFTR in brain may serve as a pathogenic mechanism for disruption of homeostasis.

PubMed Disclaimer

References

    1. Am J Physiol. 1983 Apr;244(4):R577-87 - PubMed
    1. Brain Res Mol Brain Res. 1994 Dec;27(2):329-32 - PubMed
    1. Am J Physiol. 1984 Jul;247(1 Pt 2):R173-82 - PubMed
    1. Fed Proc. 1984 Dec;43(15):2969-71 - PubMed
    1. J Cell Biol. 1985 Sep;101(3):852-61 - PubMed

Publication types

Substances