Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1995 Aug 1;14(15):3820-7.
doi: 10.1002/j.1460-2075.1995.tb00051.x.

The Saccharomyces cerevisiae translation initiation factor Tif3 and its mammalian homologue, eIF-4B, have RNA annealing activity

M Altmann  1 , B Wittmer, N Méthot, N Sonenberg, H Trachsel
Affiliations

The Saccharomyces cerevisiae translation initiation factor Tif3 and its mammalian homologue, eIF-4B, have RNA annealing activity

M Altmann et al. EMBO J. .

Abstract

The Saccharomyces cerevisiae TIF3 gene encodes the yeast homologue of mammalian translation initiation factor eIF-4B. We have added six histidine residues to the C-terminus of Tif3 protein (Tif3-His6p) and purified the tagged protein by affinity chromatography. Tif3-His6p stimulates translation and mRNA binding to ribosomes in a Tif3-dependent in vitro system. Furthermore, it binds to single-stranded RNA and catalyses the annealing of partially complementary RNA strands in vitro. In parallel experiments, RNA annealing activity could also be demonstrated for mammalian eIF-4B. A role for Tif3/eIF-4B and RNA annealing activity in the scanning process is proposed.

PubMed Disclaimer

References

    1. J Virol. 1973 Aug;12(2):241-52 - PubMed
    1. EMBO J. 1994 Aug 1;13(15):3618-30 - PubMed
    1. J Bacteriol. 1983 Jan;153(1):163-8 - PubMed
    1. J Biol Chem. 1983 Jun 10;258(11):7228-35 - PubMed
    1. Biochemistry. 1985 Oct 22;24(22):6085-9 - PubMed

MeSH terms