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. 1976 Jun;126(3):1113-8.
doi: 10.1128/jb.126.3.1113-1118.1976.

Calcium-requiring step in the uptake of deoxyribonucleic acid molecules through the surface of competent pneumococci

H Seto, A Tomasz

Calcium-requiring step in the uptake of deoxyribonucleic acid molecules through the surface of competent pneumococci

H Seto et al. J Bacteriol. 1976 Jun.

Abstract

The conversion of surface-adsorbed deoxyribonucleic acid (DNA) molecules to a state in which they are inaccessible to exogenous deoxyribonuclease requires specifically calcium ions; magnesium ions cannot replace calcium ions. Virtually maximal levels of nuclease-resistant DNA binding and genetic transformation can be obtained in media free from magnesium and containing only calcium ions. It is suggested that the calcium-requiring process is the transport of DNA molecules across the plasma membrane. Magnesium ions stimulate both the loss of surface-adsorbed DNA to the medium and the extracellular degradation of DNA.

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References

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    1. J Bacteriol. 1975 May;122(2):676-85 - PubMed
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