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. 1995 Aug 11;23(15):2811-4.
doi: 10.1093/nar/23.15.2811.

Reverse transcriptase reads through a 2'-5'linkage and a 2'-thiophosphate in a template

J R Lorsch  1 , D P Bartel, J W Szostak
Affiliations
Free PMC article

Reverse transcriptase reads through a 2'-5'linkage and a 2'-thiophosphate in a template

J R Lorsch et al. Nucleic Acids Res. .
Free PMC article

Abstract

Avian myeloblastosis virus and Maloney murine leukemia virus RNase H-reverse transcriptases pause when they encounter a 2'-5' linkage or a 2'-thiophosphate in their template RNAs, but eventually read through these backbone modifications. Both reverse transcriptases pause after the 2'-5' linkage but before the 2'-thiophosphate. These results suggest that in the absence of precise information concerning the behavior of a given reverse transcriptase with respect to a particular lesion or modification, caution should be exercised in the interpretation of primer extension data that is being used to determine the existence of, or map the position of, a crosslink, site of chemical modification or non-standard linkage in an RNA template.

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