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. 1995 Jul;36(3):649-58.

Purification and characterization of homoserine dehydrogenase from spinach leaves

S Pavagi  1 , S Kochhar, V K Kochhar, P V Sane
Affiliations
  • PMID: 7549965

Purification and characterization of homoserine dehydrogenase from spinach leaves

S Pavagi et al. Biochem Mol Biol Int. 1995 Jul.

Abstract

Homoserine dehydrogenase (HSDH) has been purified to homogeneity from spinach leaves using ammonium sulphate fractionation followed by ion exchange chromatography, gel filtration and FPLC techniques. The purified enzyme has a relative molecular mass of 220,000 and subunit molecular mass of 55,000 and probably occurs as a tetramer. The enzyme was found to be sensitive to threonine and also exhibited aspartate kinase (AK) activity, which was also sensitive to threonine suggesting that it is a bifunctional protein. The enzyme protein also gave a positive cross reaction with antibodies raised against purified AK isoenzymes. Both HSDH and AK activities were stimulated by calcium and calmodulin.

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