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. 1995 Jun 9;668(1):171-4.
doi: 10.1016/0378-4347(95)00060-v.

Thermal degradation of clozapine-N-oxide to clozapine during gas chromatographic analysis

J S Markowitz  1 , K S Patrick
Affiliations

Thermal degradation of clozapine-N-oxide to clozapine during gas chromatographic analysis

J S Markowitz et al. J Chromatogr B Biomed Appl. .

Abstract

Studies were undertaken to determine if clozapine-N-oxide, the principal urinary metabolite of the antipsychotic agent clozapine, may interfere with the gas chromatographic-mass spectrometric bioanalysis of clozapine. Following injection of clozapine-N-oxide onto a (5% phenyl)methylpolysiloxane capillary column operated at 250 degrees C, significant on-column reduction of clozapine-N-oxide to the parent drug occurred. Accordingly, preparation of biological samples for clozapine determination by gas chromatography should avoid conditions which reportedly co-extract the N-oxide to assure no artifactual contribution of this metabolite in the detection of clozapine.

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