An NSF-like ATPase, p97, and NSF mediate cisternal regrowth from mitotic Golgi fragments
- PMID: 7553851
- DOI: 10.1016/0092-8674(95)90270-8
An NSF-like ATPase, p97, and NSF mediate cisternal regrowth from mitotic Golgi fragments
Abstract
Golgi cisternae regrew in a cell-free system from mitotic Golgi fragments incubated with buffer alone. Pretreatment with NEM or salt washing inhibited regrowth, but this could be restored either by p97, an NSF-like ATPase, or by NSF together with SNAPs and p115, a vesicle docking protein. The morphology of cisternae regrown with p97 and NSF-SNAPs-p115 differed, suggesting that they play distinct roles in rebuilding Golgi cisternae after mitosis.
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