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. 1995 Jul;233(7):430-4.
doi: 10.1007/BF00180947.

Serum-induced collagen gel contraction

J Akiba  1 , A Kakehashi, N Ueno, Y Tano, B Chakrabarti
Affiliations

Serum-induced collagen gel contraction

J Akiba et al. Graefes Arch Clin Exp Ophthalmol. 1995 Jul.

Abstract

Purpose: To understand the molecular events underlying disease-related vitreous gel contraction, the effect of serum components on collagen was investigated.

Methods: Bovine vitreous or dermal collagen was incubated with a mixture of transglutaminase (TG; factor XIIIa) and fibronectin (FN), and the biochemical changes of collagen were monitored by gel electrophoresis. In addition, serum-induced changes in the volume of the collagen gel were monitored.

Results: Gel electrophoresis revealed a new high-molecular-weight band (M(r) 240,000) presumably due to intermolecular cross-links of collagen peptides and FN. The serum components also were shown to cause a significant decrease in the volume of the collagen gel. CONCLUSION. Collagen gel contraction could be attributed to the collagen-FN-collagen cross-links catalyzed by TG.

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