NADPH-diaphorase-containing cerebrovascular nerve fibres and their possible origin in the pig
- PMID: 7560908
NADPH-diaphorase-containing cerebrovascular nerve fibres and their possible origin in the pig
Abstract
NADPH-diaphorase histochemical technique was applied to demonstrate the catalytic activity of nitric oxide synthase (NOS) in the nerve fibers supplying some porcine cerebral blood vessels, as well as in ganglia thought to be their possible sites of origin. Five sexually mature Large With Polish race sows were used. The following blood vessels and their branches were studied: the basal cerebral artery, medial cerebral artery, arteries of the circle of Wills as well as arteries located in the arachnoidea. The activity of NADPH-d was visualised in whole-mount preparations from the above listed blood vessels. The presence of NADPH-d was additionally studied in the ganglia of trigeminal nerves, the sensory ganglia of vagus nerves, the pterygopalatine and cranial cervical ganglia. NADPH-d activity was found in nerve fibres supplying all the studied arteries. Larger blood vessels, the basal cerebral artery, medial cerebral artery and arteries of the circle of Wills possessed very dense NADPH-d-positive nerve plexuses while arachnoidal arteries were poorly innervated by only single nerves. The vascular nerve fibers formed bundles varying in thickness, from very thick bundles often interchanging nerve fibers to quite thin fascicles. Thick bundles were absent from the walls of medium sized vessels and small meningeal arteries where only smaller fascicles or single fibres occurred. NADPH-d-positive neurons and nerve fibres were found in all the ganglia investigated. However, pronounced differences in the number of the positive nerve structures were observed between the ganglia. In the pterygopalatine, trigeminal and sensory ganglia of the vagal nerve the vast majority of neurons were NADPH-d-positive. Numerous NADPH-d-positive nerve fibers occurred within the pterygopalatine and trigeminal ganglion while sensory ganglia of the vagal nerve comprised smaller number of fibres. Small numbers of the neurons and moderate numbers of the nerve fibres occurred in the cranial cervical ganglion. The intensity of NADPH-d reaction in the endothelium was constant independent of the size of the vessels studied.
Similar articles
-
Possible origins and distribution of immunoreactive nitric oxide synthase-containing nerve fibers in cerebral arteries.J Cereb Blood Flow Metab. 1993 Jan;13(1):70-9. doi: 10.1038/jcbfm.1993.9. J Cereb Blood Flow Metab. 1993. PMID: 7678014
-
Distribution and origins of cerebrovascular NADPH-diaphorase-containing nerve fibers in the rat.J Auton Nerv Syst. 1994 Sep;49 Suppl:S51-4. doi: 10.1016/0165-1838(94)90086-8. J Auton Nerv Syst. 1994. PMID: 7836686
-
Local NADPH-diaphorase neurons innervate pial arteries and lie close or project to intracerebral blood vessels: a possible role for nitric oxide in the regulation of cerebral blood flow.J Cereb Blood Flow Metab. 1993 Nov;13(6):978-84. doi: 10.1038/jcbfm.1993.122. J Cereb Blood Flow Metab. 1993. PMID: 8408322
-
[Migraine and nerve block].Nihon Rinsho. 2001 Sep;59(9):1717-21. Nihon Rinsho. 2001. PMID: 11554041 Review. Japanese.
-
[So-called atypical facial neuralgias].Schweiz Rundsch Med Prax. 1989 Sep 19;78(38):1018-22. Schweiz Rundsch Med Prax. 1989. PMID: 2477889 Review. German.
Cited by
-
Distribution of NADPH diaphorase-exhibiting primary afferent neurons in the trigeminal ganglion and mesencephalic trigeminal nucleus of the rabbit.Cell Mol Neurobiol. 2006 Oct-Nov;26(7-8):1265-79. doi: 10.1007/s10571-006-9079-z. Epub 2006 Jun 14. Cell Mol Neurobiol. 2006. PMID: 16773444 Free PMC article.