Spontaneous, ligand-independent activity of the cGMP-gated ion channels in cone photoreceptors of fish

Abstract

1. We studied the electrical conductance of membrane patches detached from the outer segment of single cone photoreceptors isolated from striped bass retina. 2. Only a single class of ion channels exists in the plasma membrane of the cone outer segments; they are gated by cytoplasmic cGMP and select cations over anions, but distinguish poorly among cations. In the absence of added cGMP and of divalent cations, however, membrane patches detached from the outer segments exhibit a small conductance that ideally selects cations over anions, but distinguishes poorly between Na+ and Li+. 3. The cGMP-independent conductance does not arise from the effect of residual cGMP that may remain associated with the detached membrane, because treatment of the patch with cGMP-specific phosphodiesterase does not affect this conductance. 4. The cGMP-independent conductance is pharmacologically indistinguishable from that activated by cGMP. Ca2+ and L-cis-diltiazem block both conductances at comparable concentrations and with similar quantitative characteristics. 5. We analysed the noise of Ca(2+)- or L-cis-diltiazem-dependent macroscopic currents both in the presence and in the absence of cGMP. In the presence of cGMP, the power density spectrum of the noise is well fitted by the sum of two Lorentzian components. The same function with similar corner frequencies fits the noise of the cGMP-independent currents. However, the total power in the current fluctuations is smaller in the absence of cGMP than in its presence; also, the ratio of the zero frequency asymptotes of the low over the high frequency components, S1(0)/Sh(0), is larger in the absence of cGMP than in its presence.(ABSTRACT TRUNCATED AT 250 WORDS)