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. 1995 Oct;15(10):5700-6.
doi: 10.1128/MCB.15.10.5700.

scid cells are deficient in Ku and replication protein A phosphorylation by the DNA-dependent protein kinase

N V Boubnov  1 , D T Weaver
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scid cells are deficient in Ku and replication protein A phosphorylation by the DNA-dependent protein kinase

N V Boubnov et al. Mol Cell Biol. 1995 Oct.

Abstract

Cell mutants of the Ku nuclear DNA-binding complex are ionizing radiation sensitive and show V(D)J recombination defects. Ku binds and activates a catalytic subunit of DNA-dependent protein kinase (DNA-PK), although the substrates for DNA-PK are unknown. We found that scid cell extracts were deficient in Ku phosphorylation by DNA-PK. Human chromosome 8-complemented scid cells, containing the human DNA-PK catalytic subunit, restored Ku phosphorylation. Likewise, radiation-induced RPA hyperphosphorylation was not completed in scid cells compared with control or chromosome 8-reconstituted cells. Thus, the inactivity of DNA-PK is likely responsible for the repair and recombination defects in scid cells.

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References

    1. Science. 1993 Apr 9;260(5105):207-10 - PubMed
    1. J Biol Chem. 1993 May 15;268(14):10440-7 - PubMed
    1. Mol Cell Biol. 1994 Jun;14(6):3876-83 - PubMed
    1. J Biol Chem. 1994 Jun 10;269(23):15981-4 - PubMed
    1. J Exp Med. 1985 Jan 1;161(1):18-39 - PubMed

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