Auxotrophic mutations related to symbiotic properties of Rhizobium meliloti strain L5-30

Abstract

Mutants isolated from effective R. meliloti strain L5-30 which required histidine (his-240), arginine+uracil (arg-55) and cysteine (cys-243, cys-244 and cys-246) showed also loss of effectiveness. Mutant requiring isoleucine+valine (ilv-74) was non-infective. Relation of the metabolic deficiency to the symbiotic properties of these mutants was tested comparing symbiotic response of their prototrophic revertants and transductants. It was found that all revertants and transductants of the strain his-240 were effective which suggests that histidine deficiency was the cause of their ineffectiveness. All revertants and transductants of the cysteine mutants were still ineffective. This result indicates two independent mutations which were not cotransductible. Prototrophic revertants of the mutant arg-55 were ineffective whereas 56.9 percent of transductants appeared effective suggesting close linkage of two mutations. i.e. auxotrophic and the other concerned with symbiotic effectiveness. Though one of 69 prototrophic transductants obtained from the non-nodulating mutant ilv-74 remained non-nodulating, it seems that changes in nodulating ability of the mutant are related to the auxotrophic requirements.